Centre of Applied Marine Biotechnology logo or CAMBio for short
 
  Funding Contact
Home>Research>Aquaculture and Fisheries>Current Projects>Project 1

 

Title

A comparison of molecular probe technology and morphological methods for the identification of bivalve larvae.

Objectives

Marine bivalve larvae are one of the most difficult macroscopic groups to identify at the species level, because of their size, similar shape, similar colour and because of the absence of any protruding parts to aid in their identification. The objectives of this project are the development of a technique based on sensitive, species-specific fluorescent probes called molecular beacons and real-time PCR for the detection of bivalve larvae. The project is focussed on a technique for prediction of the time, intensity and location of natural settlements of the king scallop, Pecten maximus and the common mussel, Mytilus edulis in support of the expanding shellfish aquaculture industry.
Bi-Valve Larvae

For comparative purposes, a morphological investigation of scallop and mussel larvae will also be undertaken.

Duration

2003-2006

Partners/Collaborators

None

Funding Agency

Higher Education Authority Technological Sector Research Programme - Strand III

Contact for Further Details

John Slater – Principal Investigator
Email: john.slater@lyit.ie

+ Publications/Presentations

2008 Bendezu, I.F., Slater, J.W. & Carney, B.F.
Detection of Pecten maximus and Mytilus spp. by real time PCR and molecular beacons targeting the 18S rDNA gene.
Marine Biotechnology (submitted).

2006 Bendezu I.F., Slater J.W. and Carney B.F.
Multiplex real-time PCR analysis of marine bivalve species using molecular beacons.
Presented at International Marine Genomics Conference, Sorrento, Italy, 28th October - ist November 2006

2006 Slater, J
The development and application of techniques for prediction of the scallop spatfall, Pecten maximus (L.).
J. Shellfish Res. 25 (3).

2005 Slater, J
Spawning of the scallop, Pecten maximus (L) in Mulroy Bay and the relationship with spatfall intensity.
J. Shellfish Res. 24 (4), 951-958.

2005 Slater, J
Morphological identification of larval king scallops, Pecten maximus (L) from natural plankton samples.
J. Shellfish Res. 24 (4), 937-950.

2005 Bendezu, I.F., Slater, J.W. & Carney, B.F.
Identification of Pecten maximus and Mytilus edulis/galloprovincialis by standard PCR of the 18S rDNA gene and multiplex PCR of the 16S rDNA gene.
Marine Biotechnology 7: 686-697.

2004 Bendezu, I.F., Slater, J.W. & Carney, B.F.
Development of real time PCR identification of marine bivalves.
Presented at European Aquaculture Society Conference “Biotechnologies for Quality” Barcelona, Spain 20-23rd October 2004.

2004 Bendezu, I., Slater, J., Gillespie, J., & Carney, B.
Molecular identification of bivalves by multiplex and standard PCR amplification of the 16S and 18S rDNA genes.
Presented at TecNet Marine Research Workshop, G.M I.T. 24-25th Mar 2004.

2003 Bendezu, I.F., Slater, J.W., Gillespie, J. & Carney, B.F.
Molecular identification of bivalve larvae by PCR amplification of the 16S and 18S ribosomal DNA genes and a non-organic DNA extraction method.
Presented at BioNet Conference, Galway, Ireland. 16-17th December 2003.

2003 Slater, J.W.
Forecasting the scallop spatfall using morphological identification of scallop larvae.
Presented at 14th Int. Pectinid Conference, Florida, USA. 23-29th April 2003.
 
icon
  
© CAMBio. All Rights Reserved.
  
icon