Centre of Applied Marine Biotechnology logo or CAMBio for short
 
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Title

The identification of bivalve larvae using fluorescence in-situ hybridisation (FISH) techniques.

Objectives

Biotechnological techniques for bivalve larval identification have been reported in the scientific literature, however most of the probes developed have been designed and tested only with hatchery-produced larvae, never have the probes been compared with morphological methods, nor in most cases have they been assessed for their effectiveness in the identification of mixtures of bivalve larvae from the natural plankton. This project is focussed on the development of multiplex fluorescent in-situ hybridisation (FISH) techniques for whole bivalve larvae, in which scallop larvae will be made to fluoresce one colour and mussel larvae another colour, as an aid to their identification with subsequent application of the technique to the main bivalve species under culture in Ireland.

icon Duration

2005-2008


icon Partners/Collaborators

Dr. F. Doohan, University College Dublin


icon Funding Agency

Enterprise Ireland – Applied Research Enhancement

Irish Research Council for Science, Engineering and Technology (IRCSET)


icon Contact for Further Details

John Slater, CAMBio Principal Investigator
Email: john.slater@lyit.ie

Susan Heaney, Postgraduate Researcher
Email: susan.heaney77@hotmail.com

+ Publications/Presentations

2008 Heaney, S.A., Maloy, A., Slater J.W.
Identification of bivalve larvae by fluorescence in-situ hybridisation (FISH) techniques.
Presented at 100th Centenary Conference of the National Shellfish Association, Providence, Rhode Island, U.S.A.. April 6th - 10th 2008.

2007 Heaney, S.A., Slater J.W.
The identification of bivalve larvae by fluorescence in-situ hybridisation (FISH) techniques.
Presented at 8th International Marine Biotechnology Conference, Eilat, Israel. March 11th - 16th 2007.

2006 Heaney, S., Bendezu, I.F., & Slater J.W.
The identification of bivalve larvae using FISH techniques.
Presented at International Fluorescence in-situ Hybridization Course, Vienna, Austria, 14th February 2006.

2005 Slater, J.
Morphological identification of larval king scallops, Pecten maximus (L) from natural plankton samples.
Journal of Shellfish Research 24 (4), 937-950.

2005 Bendezu, I.F., Slater, J.W. & Carney, B.F.
Identification of Mytilus species and Pecten maximus in Irish waters by PCR of the 18S rDNA gene and multiplex PCR of the 16S rDNA gene.
Marine Biotechnology 7: 686-697.

2004 Bendezu, I.F., Slater, J.W. & Carney, B.F.
Development of real time PCR identification of marine bivalves.
Presented at European Aquaculture Society Conference “Biotechnologies for Quality” Barcelona, Spain 20-23rd October 2004.

2003 Slater, J.W.
Forecasting the scallop spatfall using morphological identification of scallop larvae.
Presented at 14th Int. Pectinid Conference, Florida, USA. 23-29th April 2003.

 
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