Centre of Applied Marine Biotechnology

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Title Development of an immobilised molecular beacon DNA biosensor using electrochemical detection of hybridisation.
Objectives An electrochemical DNA biosensor, based on a molecular beacon for the mussel, Mytilus edulis (L.) immobilised onto a gold microelectrode with an electroactive compound replacing the fluorophore, will be developed. Investigation of the conditions affecting DNA absorption, the optimal time for DNA immobilization and surface coverage of immobilized DNA will be undertaken using electrochemical techniques. Electrochemical DNA biosensors, based on molecular beacons, are compatible with the requirements of a technique suitable for the development and commercialisation of “dipstick” technology for the rapid and economical testing of species-specific DNA detection. Key advantages of this hybridisation sensing method are that it is reagentless and offers potential for re-use.	Electrochemical DNA sensor
Duration 2005-2008 Partners/Collaborators Marine Institute Woods Hole Oceanographic Institution, USA Tyndall Institute, UCC Funding Agency Enterprise Ireland – Commercialisation Fund (Proof of Concept) Tyndall National Access Programme (NAP) 2008
Contact for Further Details John Slater – CAMBio Principal Investigator Email: john.slater@lyit.ie Denis McCrudden – Postgraduate Researcher Email: denis.mccrudden@lyit.ie
+ Publications/Presentations 2007 McCrudden, D., Bendezu, I.F., Slater J.W. Development of an immobilised molecular beacon DNA biosensor using electrochemical detection of hybridisation. Presented at US-Ireland R&D Partnership - Two-Day Sensors Workshop. February 20-21st 2007. 2004 McCrudden, D. Adsorption and desorption of Ru and Os complexes on Pt electrodes. Poster at the IRCSET Workshop, Dublin, 27th October 2004. 2004 McCrudden, D. Electron transfer and adsorption kinetics of some Ru and Os complexes. Presented at 8th Science Res. Colloquium, Waterford. 21-23rd May 2004. 2004 McCrudden, D. & Bendezu, I.F. Electrochemical biosensor for detection of the scallop, Pecten maximus (L.) Presented at TecNet Marine Res. Workshop, Galway. 24-25th March 2004. 2003 McCrudden, D. A study of some ruthenium and osmium complexes. Poster at the IRCSET Workshop, Dublin, 25th October 2003. 2003 McCrudden, D. Electrochemical study of some Ru and Os complexes. Presented at 7th Science Research Colloquium, Tralee. 21-23rd May 2003.

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