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Validation characteristics for the measurement
of Amnesic Shellfish Poisoning toxin. |
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Domoic acid, the toxin responsible for Amnesic
Shellfish Poisoning (ASP) has been recorded in commercially important
shellfish including scallops, mussels, oysters, clams and cockles.
The procedure for the extraction of toxin from shellfish tissue
prior to analysis involves the use of 50% aqueous methanol followed
by measurement using HPLC. For accurate work such as the preparation
of Certified Reference Material, exhaustive extraction from the
shellfish tissue is recommended, however regulatory laboratories,
under pressure to provide results, generally use a single dispersive
extraction. |
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This project
will investigate the extraction efficiency of domoic acid from a
range of shellfish tissues including the adductor muscle, hepatopancreas,
gonad and tissue remainder from the king scallop, Pecten maximus;
and from total tissue of the mussel, Mytilus edulis, the
cockle, Cerastoderma edule, the native oyster, Ostrea
edulis; the Pacific oyster, Crassostrea gigas and
the Manila clam, Ruditapes philippinarum, all commercially
important species in Europe. Extraction efficiency will be investigated
over a range of concentrations, both above and below the 20µg/g
concentration identified in EU Directive 2002/226/EC. |
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2002-2006
Marine Institute
University College Cork |
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Letterkenny Institute of Technology
Postgraduate Seed Funding
Higher Education Authority Technological Sector Research Programme
- Strand 1
John Slater – CAMBio Principal Investigator
Email: john.slater@lyit.ie
2004 Harkin, A.L., Bogan, Y., Gillespie,
J., Hess, P. & Slater, J.
The extraction efficiency of domoic acid at different concentrations
from a range of shellfish tissues.
Poster at Int. Molluscan Shellfish Safety Conf., Galway, 14-18th
June 2004.
2004 Bogan, Y., Harkin, A.L., Greenwood,
C., Gillespie, J., Hess, P. & Slater, J.
The influence of scallop size on domoic acid concentration in
the king scallop, Pecten maximus.
Poster at Int. Molluscan Shellfish Safety Conf., Galway, 14-18th
June 2004.
2004 Harkin, A.L., Bogan, Y.,
Gillespie, J., Hess, P. & Slater, J.
Extraction efficiency of domoic acid at different concentrations
in some shellfish tissues.
Presented at TecNet Marine Research Workshop, GMIT 24-25th Mar
2004.
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